1. Calcium oxalate monohydrate crystal (COM crystal) preparation
2. Techniques of lyophilization
3. Cell culture
4. Macrophage differentiation
5. Optimization of diosmin concentration in macrophage
6. Cell death assay by Annexin V-PI staining
7. MTT assay
8. Analysis of Ml and M2 macrophage markers by flow cytometry
9. Quantification of Ml and M2 macrophage cytokine by ELISA assay
10. Techniques of quantitative detection of proteins by SDS-PAGE
11. Protein tracing skills of interest with western blot technique