#B.sc. Undergraduate Thesis
Starvation potentiates the anti-cancer effect of Calotropis gigantea bark extract in colon cancer.
The purpose of this study was to investigate the effect of Calotropis gigantea bark extract in the starvation that can help inhibit colon cancer cells.
-Cell culture (HCT116 cell )
-Cell viability (MTT and Crystal violet assay)
-Cross section techniques and H&E staining (Observed)
#Apprentice
The mechanism of Pythium insidiosum-induced inflammatory response in monocytic cell line.
Objective: To determine the toll-like receptor 4-induced inflammatory response to killed hypha and live zoospore Pythium insidiosum in monocytic cell line.
- Cell cuture (THP-1 cell)
- Cultivation of microbial (Cultivation of P.insidiosum,Preparation grass blades and Cultivation of
P. insidiosum ,Preparation of live P.insidiosum zoospore,Preparation of killed P.insidiosum hypha,Killed hypha and live zoospores test )
- Gene expression by Real-time PCR(Inflamatory cytokine;IL-1B,IL6,IL8and TNF)
- Protien expression by Western blots (IL-1B,pro-caspase1,NLRP3,B-actin)